Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals
نویسندگان
چکیده
منابع مشابه
Generation of sequence signatures from DNA amplification fingerprints with mini-hairpin and microsatellite primers.
DNA amplification fingerprinting (DAF) with mini-hairpins harboring arbitrary "core" sequences at their 3' termini were used to fingerprint a variety of templates, including PCR products and whole genomes, to establish genetic relationships between plant tax at the interspecific and intraspecific level, and to identify closely related fungal isolates and plant accessions. No correlation was obs...
متن کاملUniversal Primers for Sequencing DNA Restriction
1.Atcheson, C.L., B. DiDomenico, S. Frackman, R.E. Esposito and R.T. Elder. 1987. Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene. Proc. Natl. Acad. Sci. USA 84:80358039. 2.Cha, J., W. Bishai and S. Chandrasegaran. 1993. New vectors for direct cloning of PCR products [published erratum appears in Gene 1994; 141:149]. Gene 136:369-370. 3.Clark, J.M. 19...
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BACKGROUND Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. However, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. Oligonucleotides co...
متن کاملUniversal primers for the amplification and sequence analysis of actin-1 from diverse mosquito species.
We report the development of universal primers for the reverse-transcription polymerase chain reaction (RT-PCR) amplification and nucleotide sequence analysis of actin cDNAs from taxonomically diverse mosquito species. Primers specific to conserved regions of the invertebrate actin-1 gene were designed after actin cDNA sequences of Anopheles gambiae, Bombyx mori, Drosophila melanogaster, and Ca...
متن کاملAn Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2011
ISSN: 1676-5680
DOI: 10.4238/2011.october.31.3